Sow diet prebiotic supplementation impacted ileal and jejunal PP cell numbers, but not the proportion of resident and migrating APC. Datasets were analyzed using the non-parametric Mann-Whitney test, for each age. (A) Numeration of mononuclear immune cells (×106 per g tissue) in il-PP of CTRL- (n = 4, empty black bar) and PREB (n = 4, black hatched grey bar) piglets at PND10, PND21, PND49 and PND90. (B) CD16+ MHCII+ proportion (% of total immune cells measured by flow cytometry) in CTRL- and PREB il-PP at PND10, PND21, PND49 and PND90. (C) CD16+ MHCII+ proportion (% of total immune cells) in CTRL- (n = 4, empty black bar) and PREB (n = 4, black hatched grey) jej-PP at PND49 and PND90. (D) CD11R1+ MHCII+ proportion (% of total immune cells) in CTRL- (n = 4, empty black bar) and PREB (n = 4, black hatched grey bar) il-PP at PND10, PND21, PND49 and PND90. (E) CD11R1+ MHCII+ proportion (% of total immune cells) in CTRL- (n = 4, empty black bar) and PREB (n = 4, black hatched grey bar) jej-PP at PND49 and PND90.