Skip to main content
. 2020 Jul 17;12(7):2115. doi: 10.3390/nu12072115

Figure 5.

Figure 5

Sow diet prebiotic supplementation effect on gene expression and cytokine patterns of unstimulated il-PP explants. Datasets were analyzed using the non-parametric Mann-Whitney test. (A) qRT-PCR gene expression from unstimulated il-PP explants of CTRL- (n = 3–4, full circle) and PREB (n = 3–4, empty square) piglets. The relative gene expression was determined using the cycle threshold (Ct) method and normalized to the expression of the two housekeeping genes (Ct of gene of interest over mean Ct). Differences in il-PP explant gene expression levels between CTRL- vs. PREB are displayed with * p < 0.05, # p < 0.10. (B) Cytokine secretion in unstimulated il-PP explant from CTRL- (n = 3–4, full circle) and PREB (n = 3–4, empty square) piglets. Differences in il-PP explant cytokine secretion between CTRL- vs. PREB are displayed with * p < 0.05, # p < 0.10.