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. 2020 Jul 9;20(3):3024–3034. doi: 10.3892/ol.2020.11847

Figure 3.

Figure 3.

miR-21 is a downstream target of NBAT1 in glioma. (A) The putative binding sites of miR-21 on NBAT1 transcripts. (B) Overexpression of miR-21 significantly decreased the luciferase activity of WT-NBAT1, whereas no significant changes were observed in MUT-NBAT1. (C) The RNA levels of miR-21 were increased in glioma compared with the paracarcinoma tissues. (D) The levels of miR-21 were evaluated in glioma samples using northern blotting. (E) The expression levels of miR-21 were increased in A172 and AM38 cells compared with those in A735 cells. (F) The expression of miR-21 in cell lines was assessed using northern blotting. *P<0.05. miR, microRNA; WT, wild-type; MUT, mutant; NC, negative control.