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. 2020 Jul 6;20(3):2881–2887. doi: 10.3892/ol.2020.11814

Figure 2.

Figure 2.

Multiple immunofluorescence staining for APE1, VEGFA, CD163 and CD34 in bladder cancers. (A) Representative sample with high expression of APE1 and VEGFA in which the intratumor CD163-labeled M2 macrophages are easily distinguishable. (B) Example of a sample with high expression of APE1 and VEGFA in which the CD163-labeled M2 macrophages are easily distinguishable in the tumor stroma. (C) Representative sample with low expression of APE1 and VEGFA, in which the CD163-labeled M2 macrophages are less distinguishable. APE1-positive staining in tumor epithelial cells displayed as orange and VEGFA-positive staining in tumor epithelial cells was cyan, while CD163-positive infiltrating M2 macrophages were green and CD34-positive vascular endothelial cells displayed as magenta (magnification, ×200). VEGFA, vascular endothelial growth factor A; APE1, apurinic/apyrimidinic endodeoxyribonuclease 1.