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KAR2-Induced Degradation of KAI2 Requires SMAX1 and SMXL2.
(A) Immunoblots of soluble protein isolated from seedlings of the indicated genotypes treated with 10 µM KAR2 or mock treated with 0.1% (v/v) acetone for 8 h. Total protein (60 µg) was loaded in each lane. Levels of ACTIN serve as a loading control.
(B) As per (A), except seedlings were treated with 5 µM KAR2 and ribulose-1,5-bis-phosphate carboxylase/oxygenase large subunit (RbcL) was used as a loading control.
