Figure 8.

Cytosolic Oxidation Is Only Weakly Affected by ATP and PEP1.

(A) and (B) Analyses of roots of 5-d-old Arabidopsis expressing R-GECO1 and roGFP2-Orp1 (Ca²⁺ and H₂O₂; n = 7; see [A]) in response to 1 mM ATP and 100 µM H₂O₂ and R-GECO1-P2A-roGFP2-Orp1 (Ca²⁺ and H₂O₂; n = 7; see [B]) in response to 1 µM PEP1 and 100 µM H₂O₂. Average vertical response profiles of (top) fluorescence emissions (F) or emission ratios (R) and (middle) signal changes (ΔF/F₀ or ΔR/R₀) normalized to 4-min average baseline recordings. An adjacent representative bright field (BF) root image is shown for orientation. (Bottom) Full image signal changes (mean ± sd).

Representative experiments are provided as Supplemental Movies 10 and 12. See also Supplemental Figures 6 and 7 for related data and experiments.