Figure 7.

HvCaM1 interacts with HvCAMTA4 via specific binding sites in the promoters of HKT1 genes. A, Yeast interaction assay between HvCaM1 and HvCAMTA4. B, BiFC assay between HvCaM1 and HvCAMTA4 in barley protoplasts. Bars = 5 μm. C, Expression levels of HvCAMTA4 in roots of wild-type (WT) and RNAi plants under 200 mm NaCl for 3 weeks (w). Asterisks represent significant and highly significant differences as determined by independent Student’s t test in roots of wild-type and RNAi plants (*P < 0.05 and **P < 0.01). Data are means of four replicates ± sd. D, The cis-regulatory elements of CGCG-core and CGTGT-core along 3-kb promoter regions of HKT genes, marked as red and blue, respectively. The specific sequences are enlarged in the promoters of HvHKT1;5 and HvHKT1;1.