Table 3. Anatomical characteristics in the primary leaves of soybean and French bean grown under LN and HN conditions.
LMA, CWT from TEM, leaf thickness, Sc, Sm, and the ratio of Sc/Sm from light microscopy. Values of Ct0 plants were obtained at 0 WAD, and values of Ct and Def plants were obtained at 2 WAD. Data are means (±sd), n = 4. Significant differences between Ct and Def plants were determined by Student’s t test (**, P < 0.01; *, P < 0.05; and ns, P > 0.05).
| Nitrogen Conditions | Units of Measure | Soybean | 2 WAD | French Bean | 2 WAD | ||
|---|---|---|---|---|---|---|---|
| Ct0 | Ct | De | Ct0 | Ct | De | ||
| LN | LMA (g m−2) | 29.73 ± 1.27 | 41.49** ± 8.62 | 68.40 ± 7.51 | 32.83 ± 3.75 | 25.25** ± 1.50 | 57.89 ± 10.96 |
| CWT (nm) | 129.2 ± 22.3 | 166.3** ± 34.9 | 354.1 ± 54.3 | 105.5 ± 21.7 | 99.5** ± 8.6 | 251.3 ± 71.6 | |
| Leaf thickness (μm) | 185.7 ± 18.1 | 256.2** ± 24.0 | 336.8 ± 33.4 | 183.7 ± 23.9 | 318.0 ns ± 32.4 | 410.1 ± 81.7 | |
| Sc (m2 m−2) | 13.7 ± 1.3 | 17.8** ± 1.6 | 23.0 ± 2.3 | 17.5 ± 2.6 | 10.8** ± 0.8 | 16.8 ± 2.8 | |
| Sm (m2 m−2) | 17.3 ± 1.6 | 21.4** ± 1.7 | 26.0 ± 1.7 | 19.4 ± 2.0 | 20.4** ± 3.1 | 26.1 ± 2.3 | |
| Sc/Sm (m2 m−2) | 0.79 ± 0.06 | 0.83 ns ± 0.10 | 0.88 ± 0.07 | 0.90 ± 0.05 | 0.54 ns ± 0.06 | 0.64 ± 0.08 | |
| HN | LMA (g m−2) | 26.04 ± 0.93 | 36.96** ± 2.34 | 68.11 ± 4.57 | 27.12 ± 3.04 | 36.20** ± 4.41 | 92.25 ± 14.70 |
| CWT (nm) | 112.3 ± 11.6 | 156.1** ± 33.0 | 326.4 ± 59.1 | 95.1 ± 10.3 | 101.6** ± 17.5 | 308.1 ± 29.2 | |
| Leaf thickness (μm) | 230.6 ± 15.0 | 274.3**±33.3 | 395.4 ± 16.2 | 287.7 ± 31.4 | 352.3** ± 14.9 | 545.1 ± 87.5 | |
| Sc (m2 m−2) | 18.4 ± 1.5 | 17.9** ± 2.5 | 27.9 ± 1.5 | 17.9 ± 3.0 | 16.9* ± 2.5 | 24.4 ± 5.1 | |
| Sm m2 m−2) | 19.4 ± 1.6 | 20.7* ±3.9 | 29.9 ± 1.3 | 18.2 ± 3.1 | 19.9 ns ± 2.3 | 27.5 ± 6.2 | |
| Sc/Sm (m2 m−2) | 0.95 ± 0.00 | 0.87 ns ± 0.06 | 0.93 ± 0.02 | 0.99 ± 0.00 | 0.85 ns ± 0.05 | 0.89 ± 0.08 | |