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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2020 Jan 9;34(2):3179–3196. doi: 10.1096/fj.201901777R

Figure 5. S100A6 regulation of calcium entry and barrier function is dependent upon PPP5C.

Figure 5.

A. Global calcium entry was measured in S100A6 over-expressing cells in which PPP5C expression was suppressed via siRNA. The S100A6-mediated inhibition of calcium entry following rolipram (R) + thapsigargin (TG) was reversed upon PPP5C suppression. B. Comparison of peak calcium entry observed. Peak calcium entry was increased by ~25% in cells treated with PPP5C siRNA compared to cells treated with scrambled siRNA. n = 3 independent experiments. C. ISOC was measured. The decrease in ISOC in S100A6 over-expressing cells was reversed upon PPP5C suppression as evidenced by increased current. n = 4 measurements per condition from 3 different siRNA treatments performed over two different recording periods. D. ECIS® was performed to assess the endothelial barrier. The attenuated decrease in resistance following ISOC activation in S100A6 over-expressing cells was reversed upon PPP5C suppression. E. Comparison of peak resistance decrease observed in D. The peak resistance decreases in S100A6 over-expressing cells treated with PPP5C siRNA was greater than S100A6 over-expressing cells treated with scrambled siRNA. n = 3 independent experiments.