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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: FASEB J. 2020 Jan 9;34(2):3179–3196. doi: 10.1096/fj.201901777R

Figure 6. FKBP51 regulates calcium entry and barrier function in PMVECs.

Figure 6.

A. Global calcium entry was measured in FKBP51 over-expressing PMVECs. Doxycycline-inducible FKBP51 over-expressing PMVECs revealed decreased calcium entry following rolipram (R) + thapsigargin (TG), as compared to non-doxycycline-treated cells. B. Comparison of peak calcium entry observed. Peak calcium entry was decreased by 30% in doxycycline-treated cells compared to non-doxycycline-treated cells. n = 3 independent experiments. C. ISOC was measured. Doxycycline-inducible FKBP51 over-expressing PMVECs revealed decreased ISOC following R+TG, as compared to non-doxycycline-treated cells. n = 4 measurements per condition performed over two different recording periods. D. ECIS® was performed to assess the endothelial barrier. Doxycycline-inducible FKBP51 over-expressing PMVECs revealed decreased barrier disruption following ISOC activation as compared to non-doxycycline-treated cells. E. Comparison of peak resistance decrease observed in D. The peak resistance decrease was less in doxycycline-treated cells compared to non-doxycycline-treated cells. n = 3 independent experiments.