Skip to main content
. Author manuscript; available in PMC: 2020 Aug 4.
Published in final edited form as: Microvasc Res. 2018 Dec 18;123:50–57. doi: 10.1016/j.mvr.2018.12.002

Figure 3.

Figure 3.

Quantitative RT-PCR analysis of clonally expanded iChEC-1. Clones of iChEC-1 were obtained using a limiting dilution. Comparison of iChEC-1 clones to the parent line demonstrated intact expression of endothelial cell markers across all clones. Relative expression = [(absolute copy number of the gene / absolute copies of 18s rRNA)/(copies of gene in hChEC/copies of 18s rRNA in hChEC)] . TAg = SV40 T-antigen; iChEC = immortalized choroidal endothelial cell; hChEC= primary human choroidal endothelial cells; and C= clone .