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. 2020 Jul 1;20(8):320–330. doi: 10.1002/elsc.201900147

TABLE 1.

MTX treatment on top five cell clones with highest rE2 expression level from each vector transfected cell pool

Recombinant E2 productivity/OD 450 nm a
Cell pool Top 5 clones after second G418 screening MTX 0 nM MTX 200 nM MTX 400 nM MTX 600 nM MTX 800 nM MTX 1000 nM MTX 1200 nM
CHO pCMV‐rE2 A4 0.28 ± 0.01 0.44 ± 0.02 0.59 ± 0.04 0.67 ± 0.06 0.76 ± 0.06 0.59 ± 0.06 0.47 ± 0.05
A11 0.34 ± 0.02 0.51 ± 0.01 0.58 ± 0.03 0.85 ± 0.05 0.77 ± 0.05 0.72 ± 0.01 0.69 ± 0.07
D3 0.27 ± 0.01 0.37 ± 0.05 0.51 ± 0.01 0.66 ± 0.05 0.69 ± 0.05 0.49 ± 0.04 0.42 ± 0.03
F6 0.31 ± 0.01 0.68 ± 0.03 0.61 ± 0.02 0.53 ± 0.04 0.41 ± 0.05 0.41 ± 0.06 0.31 ± 0.04
G7 0.30 ± 0.02 0.49 ± 0.01 0.59 ± 0.01 0.63 ± 0.07 0.67 ± 0.04 0.67 ± 0.07 0.39 ± 0.03
CHO pTxnip‐1‐rE2 B4 0.16 ± 0.01 0.19 ± 0.01 0.19 ± 0.01 0.20 ± 0.02 0.19 ± 0.02 0.20 ± 0.01 0.21 ± 0.01
C7 0.19 ± 0.02 0.21 ± 0.01 0.29 ± 0.02 0.25 ± 0.01 0.23 ± 0.01 0.23 ± 0.01 0.18 ± 0.02
D9 0.15 ± 0.01 0.16 ± 0.01 0.17 ± 0.02 0.18 ± 0.01 0.16 ± 0.01 0.16 ± 0.02 0.17 ± 0.02
E3 0.16 ± 0.01 0.16 ± 0.02 0.18 ± 0.01 0.20 ± 0.01 0.21 ± 0.02 0.19 ± 0.01 0.18 ± 0.01
E6 0.14 ± 0.01 0.15 ± 0.02 0.16 ± 0.02 0.16 ± 0.01 0.17 ± 0.03 0.16 ± 0.02 0.16 ± 0.01
CHO pTxnip‐2‐rE2 B2 0.19 ± 0.01 0.20 ± 0.01 0.21 ± 0.02 0.22 ± 0.01 0.19 ± 0.01 0.20 ± 0.01 0.17 ± 0.01
B6 0.19 ± 0.01 0.21 ± 0.02 0.23 ± 0.03 0.26 ± 0.03 0.23 ± 0.02 0.20 ± 0.02 0.18 ± 0.01
C11 0.18 ± 0.01 0.19 ± 0.02 0.17 ± 0.01 0.16 ± 0.02 0.16 ± 0.02 0.16 ± 0.01 0.16 ± 0.02
D8 0.18 ± 0.01 0.18 ± 0.03 0.18 ± 0.03 0.20 ± 0.03 0.21 ± 0.02 0.20 ± 0.01 0.18 ± 0.01
E8 0.21 ± 0.01 0.21 ± 0.01 0.22 ± 0.01 0.25 ± 0.02 0.23 ± 0.02 0.27 ± 0.03 0.16 ± 0.02
CHO pTxnip‐3‐rE2 B6 0.30 ± 0.01 0.33 ± 0.02 0.47 ± 0.04 0.66 ± 0.05 0.77 ± 0.08 0.67 ± 0.07 0.57 ± 0.06
C7 0.31 ± 0.01 0.48 ± 0.05 0.58 ± 0.07 0.68 ± 0.07 0.74 ± 0.09 0.88 ± 0.09 0.58 ± 0.03
D7 0.33 ± 0.03 0.55 ± 0.01 0.75 ± 0.03 0.85 ± 0.05 0.91 ± 0.02 0.89 ± 0.06 0.55 ± 0.05
F9 0.29 ± 0.01 0.37 ± 0.05 0.67 ± 0.04 0.36 ± 0.05 0.37 ± 0.02 0.27 ± 0.03 0.17 ± 0.02
G9 0.28 ± 0.01 0.39 ± 0.04 0.48 ± 0.05 0.78 ± 0.08 0.87 ± 0.10 0.58 ± 0.07 0.48 ± 0.04
CHO pTxnip‐4‐rE2 B6 0.37 ± 0.01 0.59 ± 0.04 0.67 ± 0.09 0.86 ± 0.09 0.97 ± 0.11 1.27 ± 0.13 1.13 ± 0.12
D2 0.39 ± 0.01 0.47 ± 0.05 0.58 ± 0.07 0.78 ± 0.12 0.89 ± 0.08 1.08 ± 0.11 1.01 ± 0.08
D9 0.35 ± 0.01 0.58 ± 0.03 0.67 ± 0.08 0.96 ± 0.11 1.36 ± 0.12 1.16 ± 0.09 0.96 ± 0.09
F12 0.41 ± 0.02 0.65 ± 0.04 0.98 ± 0.03 1.27 ± 0.04 1.59 ± 0.09 1.29 ± 0.07 1.01 ± 0.06
G5 0.34 ± 0.01 0.51 ± 0.05 0.67 ± 0.04 0.76 ± 0.04 0.97 ± 0.08 0.77 ± 0.07 0.57 ± 0.06
a

Results are presented as average values ± SD from three samples of each cell clone.