Fig. 2.

Drosophila 5’UTR G4C2 repeat models. a Schematic of constructs used to generate transgenic fly lines. (G4C2)28 repeats were inserted into the 5’UTR of GFP without an upstream start codon in all three reading frames relative to the GFP reading frame. b Western blot (left) of lysates from heads of G4C2 exonic repeat flies normalized to β-tubulin (right), n = 6. c Quantification of RNA foci in the retina of the indicated fly lines, n = 10. Graphs represent means ± SEM. * p < 0.05; ** p < 0.01 by Kruskal-Wallis after Dunn’s correction for multiple comparisons