Fig. 4.

G4C2 repeats toxicity is associated with nuclear accumulation of RAN translation peptides. a Representative images of transverse retinal sections from Drosophila of the indicated genotypes probed with antibody against GA dipeptide repeats. b Quantification of total GA repeat staining by immunofluorescence (left) and the percentile of cells with significant nuclear accumulation of GA DPR staining for the indicated genotypes (right). c Representative Immunofluorescence images of Drosophila retinal sections probed with antibodies against the GP dipeptide repeats. d Quantification of total GP repeat staining by immunofluorescence (left) and the percentile of cells with significant nuclear accumulation of GP DPR staining for the indicated genotypes (right). E) Representative Immunofluorescence image of transverse retinal sections from Drosophila of the indicated genotypes probed with antibody against GR dipeptide repeats. F) Quantification of total GR repeat staining by immunofluorescence (left) and the percentile of cells with significant nuclear accumulation of GR DPR staining for the indicated genotypes. Graphs represent means ± SEM. * p < 0.05; ** p < 0.01 by Kruskal-Wallis after Dunn’s correction for multiple comparisons. The number of flies analyzed for each respective genotype in B, D and F was 10