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. 2020 Jun 28;12(7):1914. doi: 10.3390/nu12071914

Figure 3.

Figure 3

Effect of probiotics on osteoblast and osteoclast differentiation. Data are presented as the mean ± SD. For osteoblast differentiation, MG-63 cells were treated with estradiol, GKM3, and GKS6 in 1% FBS medium for 7 days. Gene expression of (A) bone morphogenetic protein-2 (BMP-2), (B) alkaline phosphatase (ALP), and (C) osteocalcin (OCN) on day 7 were determined by Q-PCR. For osteoclast differentiation, RAW264.7 were treated with estradiol, GKM3, and GKS6 in the presence of 50 ng/mL RANKL for 7 days. Gene expression of (D) receptor activator of nuclear factor-κB (RANK), (E) Cellular Oncogene Fos (c-Fos), and (F) tartrate-resistant acid phosphatase (TRAP) on day 7 were determined by Q-PCR. All data were normalized to GADPH. #p < 0.05 compared with control and *p < 0.05 compared with untreated.