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. 2020 Jun 28;12(7):1914. doi: 10.3390/nu12071914

Table 1.

Specific primers used for QPCR.

Gene Forward Reverse
BMP-2 5′−GGGTTGGAACTCCAGACTGT−3′ 5′−GAAGAGTGAGTGGACCCCAG−3′
ALP 5′−CCACGTCTTCACATTTGGTG−3′ 5′−AGACTGCGCCTAGTAGTTGT−3′
OCN 5′−TGAGAGCTCTCACACTCCTCGCCCTATTGG−3′ 5′−GCTCCCAGCCATCGATACAGGTAGCGC−3′
RANK 5′−AAACCTTGGACCAACTGCAC−3′ 5′−ACCATCTTCTCCTCCCHAGT−3′
c-Fos 5′−ATGGGCTCTCCTGTCAACAC−3′ 5′−GGCTGCCAAAATAAACTCCA−3′
TRAP 5′−ACTTCCCCAGCCCTTACTACCG−3′ 5′−TCAGCACATAGCCCACACCG−3′
GAPDH 5′−ACTTTGTCAAGCTCATTTCC−3′ 5′−TGCAGCGAACTTTATTGATG−3′
BMP-2: bone morphogenetic protein-2; ALP: alkaline phosphatase; OCN: osteocalcin; RANK: receptor activator of nuclear factor-κB; TRAP: tartrate-resistant acid phosphatase