Table 4.
The short-list of lipids indicating a statistically significant difference between HM and FM samples in the different lactation stages and age range targets according to ANOVA unequal variance test (multiple testing correction: Benjamini–Hochberg, p < 0.01).
| Lipid Name | Average Peak Area Fold Change Colostrum (n = 11) vs. FM 0–6 Months (n = 8) | Average Peak Area Fold Change HM 0-6 Months (n = 10) vs. FM 0–6 Months (n = 8) | Average Peak Area Fold Change HM 6- 12 Months (n = 8) vs. FM 6–12 Months (n = 7) | Average Peak Area Fold Change HM > 12 Months (n = 16) vs. FM >12 Months (n = 5) |
|---|---|---|---|---|
| LysoPC22:6 | 12.2 a | 15.1 | ns | ns |
| PC28:0 | −5.4 | −9.6 | −5.3 | −4.8 |
| PC29:0 | −5.8 | −15.0 | −8.2 | −8.4 |
| PC31:0 | ns | −3.9 | −3.3 | −3.0 |
| PC33:0 | ns | −4.7 | −5.6 | −3.5 |
| PC33:1 | −2.7 | −5.5 | −4.1 | −2.9 |
| PC33:1 | −2.7 | −5.5 | −4.1 | −2.9 |
| PC36:4 | −11.8 | −6.3 | −6.4 | −3.7 |
| PC36:5 | −36.4 | −13.9 | −16.5 | −15.4 |
| PC38:6 | 13.6 | 5.1 | ns | ns |
| PE-O36:5 | 33.5 | 11.9 | 12.0 | 25.4 |
| PE-O38:7 | 79.9 | 26.6 | nd in FM | nd in FM |
| PI34:3 | −12.9 | nd in HM | nd in HM | nd in HM |
| SMd36:0 | 5.8 | 18.2 | 30.9 | 73.5 |
| SMd36:1 | 6.0 | 12.8 | 14.0 | 54.0 |
| SMd36:2 | 4.9 | 3.6 | 4.5 | 8.4 |
| SMd38:1 | 39.6 | 77.1 | 112.6 | 227.5 |
| SMd40:0 | −28.3 | −19.5 | −23.6 | nd in HM |
| SMd40:1_1 | 2.8 | 7.0 | 9.1 | 19.6 |
| SMd42:2 | 24.4 | 18.8 | 22.3 | 45.1 |
| SMd42:3 | 108.3 | 39.2 | ns | ns |
| SMd44:2 | 182.4 | 33.6 | ns | ns |
| TG46:1 | 2.7 | 5.8 | 5.3 | 21.3 |
| TG46:2 | ns | 6.8 | 5.3 | 44.1 |
| TG46:3 | ns | 13.1 | 7.6 | 33.8 |
| TG48:1 | 2.3 | 3.2 | 3.4 | 6.9 |
| TG48:2 | 2.7 | 5.8 | 6.7 | 11.8 |
| TG48:3 | 4.9 | 19.0 | 14.8 | 64.2 |
| TG48:4 | 4.8 | 25.9 | 16.6 | 69.5 |
| TG48:5 | 4.9 | 41.8 | 31.0 | 51.3 |
| TG50:3 | 4.7 | 9.2 | 7.9 | 13.4 |
| TG50:4 | 3.6 | 9.5 | 7.1 | 14.0 |
| TG50:5 | 4.0 | 15.3 | 12.8 | 20.4 |
| TG50:6 | 10.5 | 22.7 | ns | 54.9 |
| TG52:7 | 7.8 | 22.7 | ns | ns |
| TG56:6_1 | 53.4 | 43.8 | ns | ns |
| TG56:7_2 | 13.2 | 9.1 | 7.2 | 27.2 |
| TG56:8 | 11.8 | 12.9 | ns | ns |
| TG58:2 | −2.4 | −11.5 | −7.8 | −6.7 |
| TG58:6 | 74.1 | 31.6 | 17.1 | 20.3 |
| TG58:7_2 | 19.4 | 15.0 | 9.9 | 31.1 |
| TG58:8_1 | 45.4 | 45.3 | ns | ns |
| TG58:8_2 | 12.5 | 15.5 | ns | ns |
| TG60:2 | −3.2 | −17.6 | −8.6 | −13.8 |
a Negative fc value means higher peak area in the FM samples than in the HM samples, while positive fc value means higher peak area in the HM samples than in the FM samples. nd, not detected; ns, not statistically significant different; PC, glycerophosphocholine; PC-O, ether analogue of glycerophosphocholine; PE, glycerophosphoethanoloamine; PE-O, ether analogue of glycerophoshoethanoloamine; PS, glycerophosphoserine; PI, glycerophosphoinositol; SM, sphingomyelin; TG, triacylglycerol; TGO, ether analogue of triacyglycerol.