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. 2020 Jun 25;20(3):2021–2030. doi: 10.3892/etm.2020.8939

Figure 3.

Figure 3

Loss of miR-29b-3p rescues the effects caused by circ-ABCB10 downregulation in hAMSC-CM-treated HUVECs. (A) Scratch test and (B) Transwell assays results indicated that circ-ABCB10 downregulation on hAMSC-CM-induced HUVEC migration was largely rescued by a miR-29b-3p inhibitor. Scale bar, 100 µm. (C) Tube-formation assay results showed the effect of circ-ABCB10 downregulation on hAMSC-CM-induced HUVEC angiogenesis was significantly rescued by the miR-29b-3p inhibitor. Scale bar, 200 µm. (D) VEGFA mRNA expression levels were recovered when HUVECs were co-transfected with miR-29b-3p inhibitor. (E) VEGFA protein expression levels and ERK1/2 activation were rescued by miR-29b-3p inhibitor. (F) circ-ABCB10 expression levels in hAMSC-CM were unchanged after incubation with HUVECs for 24 h. *P<0.05. HUVECs, human umbilical vein endothelial cells; hAMSCs, human amnion-derived mesenchymal stem cells; CM, conditioned medium; CM, hAMSC-CM; CM-aft, hAMSC-CM incubated with HUVECs for 24 h; VEGF, vascular endothelial growth factor; p-, phosphorylated; MSCs, mesenchymal stem cells; siRNA, small interfering RNA; circ, circular RNA; inh, inhibitor.