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. 2020 Jun 16;103(2):343–356. doi: 10.1093/biolre/ioaa093

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© The Author(s) 2020. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of compound 25 on sperm motility after administration to rats and on in vitro fertilization. (A) Compound 25 was administered to rats by oral gavage at a dose of 5 mg/kg of body weight for 3 days. Then sperm motility was measured on sperm collected from the epididymis of the rats. (B) Effect of compound 25 on in vitro fertilization assays. Epididymal sperm was pretreated for 1 h with or without 10 nM of compound 25. After placing sperm in contact with oocytes from hyper-ovulated rats, cultures were maintained for 24 h, and fertilization was estimated by the presence of the development of two cell embryos. Values are mean ± SEM.