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. 2020 Apr 25;103(2):235–243. doi: 10.1093/biolre/ioaa059

Figure 3.

Figure 3

Cib4–7/−7 mice are infertile with small testis size. (A) Testes of Cib4+/−7 and Cib4–7/−7 mice. Scale bar: 2 mm. (B) The average testis weight (one testis) per body weight of Cib4+/−7 and Cib4–7/−7 mice. **P < 0.01, Student t-test, error bars represent SD. (C) PAS staining of testes cross-sections. In stage VII–VIII seminiferous tubules, normal round spermatids (black arrowheads) were observed; however, elongated spermatids (white arrowhead) were hardly found in Cib4–7/−7 testis. Multinucleated cells (white arrow) were frequently detected in the lumen of seminiferous tubules. Black rectangles indicate magnified regions. (D) PAS staining of cauda epididymis. Round spermatid-like cells (black arrow) and multinucleated cells (white arrow) were observed in Cib4–7/−7 epididymis. Black rectangles indicate magnified regions. Twelve-week-old mice were used for A–D.