Figure 1.

Antibody production in response to immunization with elastin or oxidized elastin. ELISA analysis was performed, coating plates with elastin or oxidized elastin peptide. Serum at different concentrations (1:100 to neat) from animals immunized with elastin or oxidized elastin was used to probe for binding, which was visualized with corresponding anti-mouse IgG and IgM secondary antibodies. Values were background subtracted and averaged (n = 3). After immunization and smoke exposure, a significant immune response against ox-elastin could be detected, based on IgG and IgM binding, whereas the response against control elastin was more modest.