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. 2020 Apr 16;61(4):15. doi: 10.1167/iovs.61.4.15

Figure 1.

Figure 1.

Anti-inflammatory and anti-angiogenic effects of PEA in OIR. (A) Representative images of western blotting for TNF-α and ICAM1 in OIR retinas treated with VEH or PEA. Protein levels of (B) TNF-α and (C) ICAM-1 were quantified by densitometry and normalized to β-actin levels (n = 6). (D) Representative images of western blotting for VEGF in OIR retinas treated with VEH or PEA. (E) Protein level of VEGF was quantified by densitometry and normalized to β-actin levels (n = 6). (F) Quantitative RT-PCR of Vegfa mRNA expression in VEH-OIR and PEA-OIR groups (n = 5). (G) Retinas from VEH- and PEA-treated OIR mice were flat-mounted and stained with isolectin GS-IB4 (red). Areas of (H) retinal vaso-obliteration and (I) neovascularization were quantified in VEH- and PEA-treated OIR retinas (n = 8). (J) Retinal cryosections from VEH- and PEA-treated OIR mice were stained with TUNEL (green) and 4′,6-diamidino-2-phenylindole (DAPI) (blue). Representative images are shown. (K) Apoptotic cells were quantified and compared in VEH- and PEA-treated OIR mice. Data are presented as mean ± SEM; **P < 0.01, ***P < 0.001.