Table 3.

Microbiological and anatomopathological findings of the studied dogs.

Group	Sex	Clinical Background	Pathological Findings	Virological Examination	Bacteriological Examination	Cause of Death
1	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, hemorrhagic gastroenteritis	Canine parvovirus type 2a (wild type)—detected in the lung, liver, heart, brain, and intestine	Streptococcus dysgalactiae—isolated in the lung	Viral infection
	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, severe broncho-pneumonia, catarrhal enteritis	No viruses detected	Streptococcus sanguinis—isolated in the intestine	Undetermined: severe pneumonia due to unexplained causes
	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, severe broncho-pneumonia, catarrhal enteritis	No viruses detected	Streptococcus sanguinis—isolated in the intestine	Undetermined: severe pneumonia due to unexplained causes
	F	Acute respiratory insufficiency	Visceral congestion, multifocal pulmonary hemorrhages, hemorrhagic enteritis	Canine parvovirus type 2b and 2c (wild type)—detected in the lung, liver, spleen, heart, and intestine	Clostridium perfringens, Detection of Clostridium perfringens alpha toxin—isolated in the intestine	Viral infection
	M	Acute respiratory insufficiency	Lobar pneumonia, pulmonary edema, catarrhal enteritis	Canine distemper virus—detected in the lung, liver, and brain	Pasteurella spp.—detected in the lung	Viral and bacterial infection
	F	Acute respiratory insufficiency	Visceral congestion, multifocal pulmonary hemorrhages, hemorrhagic enteritis	Canine parvovirus type 2b and 2c (wild type)—detected in the lung, liver, brain, heart, and intestine	E. coli, Clostridium perfringens, Detection of Clostridium perfringens alpha toxin—isolated in the intestine	Viral infection
	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, focal broncho-pneumonia, hemorrhagic gastroenteritis	Canine distemper virus—detected in the lung, liver, and brain	Pasteurella spp.—detected in the lung	Viral and bacterial co-infection
	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, hemorrhagic gastroenteritis	No viruses detected	No bacteria detected	Undetermined
	M	Acute respiratory insufficiency	Visceral congestion, pulmonary edema, severe broncho-pneumonia	No viruses detected	No bacteria detected	Undetermined
	M	Acute respiratory insufficiency	Visceral congestion, hemorrhagic enteritis	Canine parvovirus type 2a (wild type)—detected in the heart, spleen, and intestine	No bacteria detected	Viral infection
3	M	Sialorrhea, unilateral eye swelling, muscle stiffness, a single episode of vomiting	Visceral congestion, pulmonary edema, hemorrhagic enteritis	No virus detected	E. coli; Clostridium perfringens Detection of Clostridium perfringens alpha toxin—detected in the intestine and lung	Bacterial infection
	M	Neurological symptoms	Visceral congestion, bi-lateral pneumonia, pulmonary edema segmental catarrhal enteritis	Rotavirus (detected in the intestine)	E. coli—isolated in the intestine	Undetermined: severe pneumonia due to unexplained causes
	F	Acute gastrointestinal symptoms	Visceral congestion, hemorrhagic enteritis, focal pneumonia	Canine parvovirus type 2a, (wild type)—detected in the lung, liver, and intestine; Adenovirus—detected in intestine	Clostridium perfringens Detection of Clostridium perfringens alpha toxin—isolated in the intestine	Viral infection
	F	Vomiting	Visceral congestion, hemorrhagic enteritis, focal pneumonia	Canine parvovirus type 2a (wild type)—detected in the lung, liver, heart, and intestine	E. coli—isolated in intestine and lung	Viral and bacterial co-infection
	M	Vomiting	Visceral congestion, pulmonary congestion, enteritis, abdominal, thoracic and pericardial effusion, multifocal pulmonary hemorrhage	No virus detected	E. coli—isolated in intestine	Undetermined: insufficient findings to explain death
4	F	Acute respiratory insufficiency	Multifocal hemorrhage, abdominal, thoracic and pericardial effusion, hemorrhagic enteritis	No virus detected	E. coli—detected in the liver, lung, and intestine Clostridium perfringens Detection of Clostridium perfringens alpha toxin—detected in the intestine and lung	Bacterial infection
5	M	Acute respiratory insufficiency	Pulmonary congestion, segmental catarrhal enteritis	Canine parvovirus type 2a (wild type)—detected in lung, liver, and intestine	E. coli—isolated in the intestine	Viral infection
	F	A single episode of diarrhea	Pulmonary congestion, pulmonary edema, segmental hemorrhagic enteritis	Canine parvovirus type 2a (wild type)—detected in lung, liver, intestine, and spleen Adenovirus—detected in the lung	No bacteria detected	Viral infection
6	F	Lack of appetite	Thoracic effusion, visceral congestion, multifocal hemorrhage, severe hemorrhagic enteritis	Canine parvovirus type 2a (vaccinal) and 2c (wild type)—detected in lung, liver, intestine, and spleen Canine distemper virus-detected in lung Adenovirus—detected in lung	No bacteria detected	Viral infection
	F	Lack of appetite and fever for 12 h	Multifocal hepatic necrosis, hemorrhagic enteritis	Canine parvovirus type 2b (wild type)—detected in the lung, liver, brain, and intestine	E. coli. Clostridium perfringens, Detection of Clostridium perfringens alpha toxin (isolated in lung, liver, and intestine)	Viral and bacterial infection
	F	Single episode of diarrhea	Congestion of the spleen, abdominal effusion	No viruses detected	No bacteria detected	Undetermined: insufficient findings to explain death