Table 1. Summary of microtubule dynamics and microtubule interactions at different concentrations of HIS-TAN1.
| Plus-end dynamics | 0 µM HIS-TAN1 | 0.1 µM HIS-TAN1 | 0.5 µM HIS-TAN1 | 1 µM HIS-TAN1 | 2 µM HIS-TAN1 |
|---|---|---|---|---|---|
| Growth events (n) | 156 | 180 | 166 | 214 | 196 |
| Growth rate (μm/s, mean ± SD) | 1.8 ± 0.4 | 1.8 ± 0.3 | 1.8 ± 0.3 | 1.7 ± 0.5* | 1.5 ± 0.3*** |
| Shrinkage events (n) | 109 | 127 | 113 | 153 | 149 |
| Shrinkage rate (μm/s, mean ± SD) | 31.5 ± 15.6 | 27.7 ± 10.8 | 26.2 ± 8.8* | 27.8 ± 9.7 | 24.2 ± 10.0*** |
| Time growing (%) | 94.9 | 93.8 | 94.5 | 94.6 | 95.2 |
| Time shrinking (%) | 5.1 | 6.2** | 5.5* | 5.4 | 4.8 |
| Catastrophe frequency (events/min) | 0.3 | 0.4 | 0.4 | 0.4 | 0.5 |
| Crossovers (n) | 445 | 346 | 334 | 334 | 506 |
| Bundling events (n) | 2 | 0 | 0 | 3 | 139 |
| Bundling frequency (%) | 0.5 | 0 | 0 | 0.9 | 27.5 |
Bundling includes both zippering and pulling. Significance was calculated by comparing values to 0 µM HIS-TAN1 determined by Mann–Whitney test (*, P > 0.05; **, P > 0.01; ***, P > 0.001). Three trials were performed for each concentration of HIS-TAN1.