Figure 3.

Specific inhibition of HDAC6 protects against MT disorganization. (A) Schematic representation of the experimental time course. (B and C) 4-d-old C2C12 myotubes were pretreated with the specific HDAC6 inhibitor TubA (5 µM) or with DMSO (CTL; 1 µl). After 24 h of pretreatment, cells were either treated for 1 h with nocodazole (10 µM) or put on ice for 6 h (cold treatment). C2C12 cells were stained with an antibody against acetylated tubulin (ac-tubulin, in red). (D) Quantification of the distribution of the organization of the MT network in myotubes (three independent experiments for each condition; n = number of myotubes counted; between 41 and 71 myotubes). Means ± SEM. ***, P < 0.001; Mann-Whitney U test. (B and C) Scale bars: 200 µm; inset magnifications (zoom): 50 µm. ac-tub, acetylated tubulin.