Figure S1.

Expression of tubulin acetylated via HDAC6 inhibitors in HEK293 cells. HEK293 cells were treated for 24 h with different HDAC6 inhibitors at different concentrations: TubA (0.05, 0.5, and 5 µM), HPOB (0.05, 0.5, and 5 µM), ACY-1215 (0.01, 0.1, and 0.5 µM), and TBC (0.05, 0.5, and 5 µM) with a pan-HDAC inhibitor: TSA (0.001, 0.01, and 0.1 µM); or with DMSO (Ctrl, control; 0.1, 1, and 10 µl). Representative Western blots showing acetylated tubulin (acetyl-tubulin) and β-tubulin expressions. GAPDH was used as a loading control. Mr(K), relative molecular weight in kiloDalton.