Figure S4.

Effects of tubA treatment on synaptic gene RNA levels and in vivo gait measurements. (A) Overview of the NMJ-morph platform. Flowchart demonstrating the sequence of analyses for each NMJ using NMJ-morph (adapted from Jones et al., 2016). NMJs were stained with α-BTX–A488 (in green). Bar: 25 µm. (B) Endogenous relative levels of AChR α-subunit, AChR β-subunit, AChR ε-subunit, MuSK, LRP4, Dok-7, and rapsyn mRNA as determined by quantitative RT-PCR in TA and DIA muscles. Three reference genes (GAPDH, 18S, and actin) were used to normalize data. (C) DigiGait analysis. Gait measurements such as swing time, stance time, or stride time were performed. (D) Horizontal ladder. The numbers of successful steps (hit), slips/missed steps, partial steps, and cheat steps were quantified. n = number of mice used per condition (veh = 15, and TubA = 14). Graphs show means ± SEM. Mann-Whitney U test. veh, vehicle.