Video 1.
Dynamics of AChR clusters with HDAC6 inhibitors in C2C12 cells. 4-d-old myotubes were treated either with TubA (5 µM), TSA (0.1 µM), or a vehicle (DMSO, 1 µl). AChR clusters were labeled with α-BTX–A488 (in green) as described in Fig. 4 E. Using cell live imaging acquisition, AChR clusters were imaged every 30 min for 12 h at 37°C on an IncuCyte ZOOM system. In six-well plates, 16–25 images were taken by well per condition. Imaging was performed every 30 min for 12 h. The video represents 24 images in 5 sec.