Table 1. Antibodies.
Antibody name | Type | Dilution | Provider | |
---|---|---|---|---|
Immunostaining | Western blotting | |||
βTub 2.1 (tubulin) | Mouse monoclonal | 1:500 | 1:10,000 | Sigma, clone TUB2.1, #T5201 |
αTubulin (11H10) | Rabbit monoclonal | 1:100 | 1:1,000 | Cell Signaling, #2125 |
Acetylated tubulin | Mouse monoclonal | 1:200 | 1:2,000 | Sigma, clone 6-11 B-1, #T7451 |
HDAC6 | Rabbit polyclonal | 1:4,000 | Sigma, #SAB4500011 | |
HDAC6 (D21B10) | Rabbit monoclonal | 1:400 | 1:4,000 | Cell Signaling, #7612 |
Paxillin (Y113) | Rabbit monoclonal | 1:500 | 1:10,000 | Abcam, #ab32084 |
Paxillin (5H11) | Mouse monoclonal | 1:100 | Invitrogen, #MA5-13356 | |
Myosin Heavy Chain sarcomere | Mouse monoclonal | 1:200 | DSHB, #MF20 | |
Normal mouse IgG | Mouse monoclonal | 1:100 | DSHB, #MF20 | |
GFP | Mouse monoclonal | 1:1,000 | Roche, #11 814 460 001 | |
GAPDH (HRP) | Goat polyclonal | 1:20,000 | Abcam, #ab85760 | |
C6orf134 (tubulin acetyltransferase) | Rabbit polyclonal | 1:200 | 1:2,000 | Abcam, #ab58742 |