Skip to main content
. 2020 Jul 28;11:1569. doi: 10.3389/fimmu.2020.01569

Figure 6.

Figure 6

CD138+ T cells exhibit central memory T cell phenotype. (A,B) Splenocytes were collected from 12 weeks old MRL/Lpr mice. (A) The expression of CD44 and CD62L on TCRβ+CD138- and TCRβ+CD138+ cells were measured by flow cytometry. Representative pseudocolor plots are shown. Mean percentages ± SD of five mice from two separate experiments are plotted. (B) Representative flow cytometry histogram of CCR7 expression on TCRβ+CD138- and TCRβ+CD138+ cells are shown. Mean ± SD percentages and MFIs for CCR7 expression from six mice in two separate experiments are plotted. (C) Splenic TCRβ+CD138+ and TCRβ+CD138- cells were sorted from 12 weeks old MRL/Lpr mice and BCL-6, Bim1, and Prdm1 mRNA were quantified by Q-PCR. Mean ± SD values of five mice from two separate experiments are plotted. (D) Splenic TCRβ+CD138+ and TCRβ+CD138- cells were sorted from 12 weeks old MRL/Lpr mice and cultured with anti-CD3/CD28 antibodies overnight. Culture supernatant IFNγ and IL-2 levels were measured by ELISA. Mean ± SD of nine mice from three independent experiments are plotted. (E) Splenic TCRβ+CD138+, TCRβ+CD138- cells were sorted from 12 weeks old MRL/Lpr mice and co-cultured with sorted B cells from the same mice in the presence of DNA for 3 days. Culture supernatant IFNγ and IL-2 levels were measured by ELISA. Mean ± SD of five mice from two separate experiments are plotted. (F) Cells were collected from spleen, lymph nodes, and bone marrows of 12 weeks old MRL/Lpr mice. Frequencies of Tcm (CD44+CD62L+) and Tem (CD44+CD62L-) cells among TCRβ+CD138- and TCRβ+CD138+ cells were measured in flow cytometry. Mean percentages ± SD of five mice from two independent experiments are plotted. (G) Splenocytes were collected from 12 weeks old Balb/c, C57BL/6, and MRL/Lpr mice. Frequencies of Tcm (CD44+CD62L+) and Tem (CD44+CD62L-) cells among TCRβ+CD138- and TCRβ+CD138+ cells were measured in flow cytometry. Mean percentages ± SD of five mice from two independent experiments are plotted. Two tailed Mann-Whitney rank sum test was used to calculate statistical significance.