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. 2020 Jul 13;20(3):2691–2697. doi: 10.3892/etm.2020.8996

Figure 4.

Figure 4

Gene silencing of MALAT1 suppresses the protein expression levels of α-SMA and fibronectin in HFL1 cells. (A) siMALAT1 transfection inhibited the expression levels of α-SMA and fibronectin protein, whereas TGF-β promoted the expression levels of α-SMA and fibronectin protein. (B) The relative expression levels of α-SMA and fibronectin normalized to GAPDH, and the ratio of P-S6K1 (T389) to total S6K1 formalized to GAPDH. GAPDH was used as a loading control and for normalization. *P<0.05 and ***P<0.001 vs. siControl; #P<0.05 vs. siControl + TGF-β group. α-SMA, α-smooth muscle actin; MALAT1, metastasis-associated lung adenocarcinoma transcript 1; p, phosphorylated; S6K1, mTOR complex 1 S6 kinase; si, small interfering RNA; T, total; TGF-β, transforming growth factor-β.