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. 2020 Jul 13;20(3):2870–2878. doi: 10.3892/etm.2020.8999

Figure 2.

Figure 2

Lyc protects against HG-induced MPC5 podocyte apoptosis. MPC5 podocytes were divided into the following groups: i) NG (5.5 mM glucose); ii) HG (25 mM glucose); iii) HG + L-Lyc (25 mM glucose + 3.125 mM Lyc); iv) HG + H-Lyc (25 mM glucose + 12.5 mM Lyc); and v) H-Lyc (12.5 mM Lyc). The rate of MPC5 podocyte apoptosis was (A) determined by flow cytometry and (B) quantified. The protein expression levels of antiapoptotic (Bcl-2) and proapoptotic (Bax and C caspase-3) proteins were (C) determined by western blotting and (D) semi-quantified. **P<0.01 vs. NG; #P<0.05 and ##P<0.01 vs. HG; ∆P<0.05 and ∆∆P<0.01 vs. H-Lyc. Lyc, lycopene; NG, normal glucose; HG, high glucose; L-Lyc, low-concentration lycopene; H-Lyc, high-concentration lycopene; C caspase-3, cleaved caspase-3; PI, propidium iodide.