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. 2020 Jul 13;20(3):2870–2878. doi: 10.3892/etm.2020.8999

Figure 3.

Figure 3

Lyc enhances PI3K/AKT activation and inhibits HG-induced MPC5 podocyte apoptosis. MPC5 podocytes were divided into the following groups: i) NG (5.5 mM glucose); ii) HG (25 mM glucose); iii) HG + H-Lyc (25 mM glucose + 12.5 mM Lyc); iv) HG + LY (25 mM glucose + 20 µM LY); and v) HG + H-Lyc + LY (25 mM glucose + 12.5 mM Lyc + 20 µM LY). Protein expression levels of p-PI3K, PI3K, p-AKT and AKT were (A) determined by western blotting and (B) quantified. The ratios of (C) p-PI3K/PI3K and (D) p-AKT/AKT. The rate of apoptosis was (E) determined by flow cytometry and (F) quantified. Protein expression levels of nephrin and podocin were (G) determined by western blotting and (H) quantified. **P<0.01 vs. NG; #P<0.05 and ##P<0.01 vs. HG; ^P<0.05 and ^^P<0.01 vs. HG + H-Lyc; †P<0.05 and ††P<0.01 vs. HG + LY. Lyc, lycopene; NG, normal glucose; HG, high glucose; H-Lyc, high-concentration lycopene; LY, LY294002; p, phosphorylated; PI, propidium iodide.