Figure 3. Contaminating cells increase basal glycolysis.

Total CD4⁺ T cells were purified from naïve mice using CD4 beads or the Tyto and then seeded into a 96 well Seahorse plate. Cells were pooled from 5–10 mice per group with three to six technical replicates per group. A) The extracellular acidification rate (ECAR) was measured after activation in real-time with anti-CD3/CD28 beads and subsequent injection of oligomycin and 2-DG. B) The average basal glycolysis rate was determined. Panel A is representative of 2–3 independent experiments and panel B is pooled from 2–3 independent experiments. Error bars represent SEM. * p ≤ 0.05.