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RT-qPCR thermal cycling conditions.
| Step | CDC(55) | Georgia Tech |
|---|---|---|
| (1) UNG incubation | 25°C, 2 min | ---- |
| (2) Reverse transcription | 50°C, 15 min | 50°C, 15 min |
| (3) RT inactivation and/or DNA polymerase activation | 95°C, 2 min | 95°C, 5 min |
| (4) Denaturation | 95°C, 3 s | 95°C,15 s |
| (5) Annealing and extension (fluorescence collection) | 55°C, 30 s | 55°C, 30 s |
| Number cycles of steps 4 and 5 | 45 | 45 |
