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. 2020 Jul 9;9(7):599. doi: 10.3390/antiox9070599

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Tubastatin A on thioredoxin-1 expression and activity. (A) Western analysis of thioredoxin-1 (Trx-1) protein expression measured in retinas of STZ-rats (DB), STZ-rats receiving TS (10 mg/kg) (DB + TS) and normoglycemic control rats (control). (B) Quantification of optical density of Trx-1 immunoblotting normalized versus actin. (C) Trx-1 enzymatic activity measured in STZ-rats (DB), STZ-rats treated with TS (10 mg/kg) (DB + TS). Values are mean ± SEM for n = 6. * p < 0.01 vs. control and # p < 0.01 vs. DB.