Table 2.
Phenolic derived metabolites identified in urine.
| Metabolites | RT (min) | Experimental m/z | MS/MS Fragments | Occurrence | |
|---|---|---|---|---|---|
| P + HFD | PECH + HFD | ||||
| Epicatechin sulphate | 5.2 | 369.0286 | 125.0235; 109.0289; 289.0708 | + | + |
| 5-(3′,4′-Dihydroxyphenyl-γ-valerolactone | 5.4 | 207.0663 | 163.0764; 122.0369 | + | + |
| 5-(3′,4′-Dihydroxyphenyl-γ-valerolactone 3′-sulphate | 3.9; 5.3 | 303.0180 | 223.0605; 179.0702 | + | + |
| Ferulic acid 4-sulphate | 8.3 | 273.0074 | 193.0505; 134.0379 | + | − |
| Feruoylquinic acid glucuronide | 5.5 | 543.1355 | 367.1013; 498.9785; 173.0450 | + | − |
| Hydroxytyrosol glucuronide | 3.0 | 329.0878 | 123.0445; 153.0169; 176.0381; 131.0349 | + | + |
Analytical parameters for the determination of phenolic-derived metabolites in urine samples using UPLC-ESI-QTOF-MS. The different diets compared were phenolic-enriched high-fat diet (P+HFD) and lyophilized phenolic enriched cooked ham enriched high-fat diet (PECH+HFD, n = 8).