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Biochemical parameters of WT and E319A mutant TbASCT.
| Acetyl-CoA |
Succinate |
|||
|---|---|---|---|---|
| Km (mM) | kcat (s−1) | Km (mM) | kcat (s−1) | |
| WT | 0.046 ± 0.007 | 104 ± 3.91 | 13.8 ± 0.474 | 191 ± 4.25 |
| E319A | N.D. | N.D. | N.D. | N.D. |
Values represent the average ± SE (n = 3) calculated using GraphPad Prism 7. N.D. indicates non-detectable level.
