Figure 2.

Honey extracts protect HaCaT cells from UVB-induced DNA damage. Cells were pre-incubated with 20 μg/mL of ME16.1 (a) or ME20.3 (b) for 2 h and then were either UVB-irradiated (55 mJ/cm²; treated) or left untreated (control). Treated and control cells were further incubated for 2 h in the presence or absence of honey extracts, left to recover for 24 h and then subjected to single cell gel electrophoresis (comet) assay. Data represent the fold-change of DNA damage in untreated (white), UVB-irradiated (black), honey-treated (light grey) and UVB-irradiated/honey-treated cells (dark grey). Data presented are the mean ± SD of three independent experiments performed in duplicates. ## p ≤ 0.01, #### p ≤ 0.001, significant differences compared to untreated cells * p ≤ 0.05, significant differences between UVB-irradiated and honey-treated/UVB-irradiated cells.