Figure 4.

Effect of polyphenols on the expression of genes encoding redox enzymes and nuclear factor erythroid 2-related factor 2 (Nrf2) transcription factor on cerebral endothelial cells in hyperglycemic condition. Cells were exposed to normoglycemic condition (NG) or hyperglycemic condition (HG) in the presence or not of each polyphenol-rich plant extract (10 µM GAE) or standard polyphenol (10 μM). The expression of genes coding for Cu/ZnSOD (A), MnSOD (B), catalase (C), and heme oxygenase-1 (HO-1) (D), GPx (E), Nox4 (F), and Nrf-2 (G) was measured by RT-qPCR and normalized to GAPDH gene expression. The data are means ± SEM of three independent experiments. *: p < 0.05, **: p < 0.01 and ***: p < 0.005, as compared to NG. #: p < 0.05, ##: p < 0.01 and ###: p < 0.005 as compared to HG.