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. 2020 Jul 17;9(7):633. doi: 10.3390/antiox9070633

Figure 4.

Figure 4

AZD0364 and ZSTK474 enhanced reactive oxygen species (ROS) production and affected reduced glutathione/oxidized glutathione (GSH/GSSG) ratio in leukemia cells. REH, MOLT-4 and MOLM-14 cells were incubated for 48 h with AZD034 (16 µM for REH, 2 µM for MOLT-4 and MOLM-14 cells) and ZSTK474 (4 µM for REH, 0.5 µM for MOLT-4 and MOLM-14 cells) alone or in combination. (a–c) Representative histograms of ROS-negative (M1) and ROS-positive (M2) cells and graphs showing the percentage of ROS-positive cells. (d) The relative GSH/GSSG ratio for untreated control and AZD0364 and ZSTK474 treated REH, MOLT-4 and MOLM-14 cells. Results represent the net luminescence (relative luminescence units, RLU). Each value is the mean  ±  SD of three independent experiments Significantly different at: * (p  <  0.05), ** (p  <  0.01) vs control; # (p  <  0.05), ## (p  <  0.01) vs. AZD0364; $ (p  <  0.05), $$ (p  <  0.01) vs. ZSTK474.