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Identity, code unit, coded levels and actual experimental values of each variable used for UAE of TFC from N. lotus stamens.
| Variable | Code Unit | Coded Variable Levels | ||
|---|---|---|---|---|
| −1 | 0 | +1 | ||
| Ethanol concentration (% v/v) 1 | X1 | 50 | 75 | 100 |
| US frequency (kHz) | X2 | 0 | 22.5 | 45 |
| Extraction duration (min) | X3 | 20 | 40 | 60 |
1 Percentage of ethanol (analytical grade) concentration in mixture with ultrapure water (HPLC grade).
