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. 2020 Jul 6;9(7):592. doi: 10.3390/antiox9070592

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glo1 levels in ventricular tissues. (A) shows immuno-fluorescence staining for Glo1 in myocytes and cSMCs from the control, T1DM, AAV2/9-Endo-eGFP-treated T1DM, and AAV2/9-Endo-Glo1-treated DM rats. White arrow emphasizes the diffuse staining associated with eGFP expression. Graphs in (B) and (C) are mean ± S.E.M. for n ≥ 30 sections obtained from N = 4–6 animals per group. (D) shows an autoradiogram for Glo1 protein in hearts from the control, AAV2/9-Endo-eGFP treated control, T1DM, AAV2/9-Endo-eGFP-treated T1DM, and AAV2/9-Endo-Glo1-treated T1DM rats. Graph below are mean ± S.E.M from ventricular homogenates from n = 5 rats done in duplicates. * denote significantly different from the control (p< 0.05), # denote significantly different from T1DM (p < 0.05). Bar at bottom of each image = 50 µm.

Glo1 levels in ventricular tissues. (A) shows immuno-fluorescence staining for Glo1 in myocytes and cSMCs from the control, T1DM, AAV2/9-Endo-eGFP-treated T1DM, and AAV2/9-Endo-Glo1-treated DM rats. White arrow emphasizes the diffuse staining associated with eGFP expression. Graphs in (B) and (C) are mean ± S.E.M. for n ≥ 30 sections obtained from N = 4–6 animals per group. (D) shows an autoradiogram for Glo1 protein in hearts from the control, AAV2/9-Endo-eGFP treated control, T1DM, AAV2/9-Endo-eGFP-treated T1DM, and AAV2/9-Endo-Glo1-treated T1DM rats. Graph below are mean ± S.E.M from ventricular homogenates from n = 5 rats done in duplicates. * denote significantly different from the control (p< 0.05), # denote significantly different from T1DM (p < 0.05). Bar at bottom of each image = 50 µm.