Table 2.
GSH:GSSG ratios and activities of γ-glutamylcysteine ligase and glutathione reductase.
| Parameters Measured |
Control (n = 6) |
T1DM (n = 6) |
AAV2/9-Endo-eGFP-Treated T1DM (n = 6) |
AAV2/9- Endo-Glo-I-Treated T1DM (n = 6) |
|---|---|---|---|---|
| GSH:GSSG ratio | 2.4 ± 0.2 | 1.55 ± 0.1 * | 1.6 ± 0.3 * | 2.3 ± 0.3 # |
|
γ-glutamylcysteine ligase activity
(mU/mg protein) |
470.5 ± 18.1 | 205.2 ± 1.2 * | 210.5 ± 21.2 * | 460.2 ± 20.4 # |
|
glutathione reductase activity
(mU/mg protein) |
48.1 ± 7.4 | 32.5 ± 8.0 * | 30.5 ± 11.2 * | 45.5 ± 10.1 # |
Values shown are mean ± S.E.M. for n = 4–6 animals per group. * denotes significant difference from Con (p < 0.05), # denotes significant difference from T1DM (p < 0.05).