Figure 8.

(A) MTT viability of PDPCs cultured on PEGDA-AA Ann. and PEGDA-AA/Arb AE Ann. and exposed to oxidative stress for up to 7 days of culture. Data are expressed as a percentage of PEGDA-AA Ann.; * p < 0.05 vs. all conditions. (B) Histogram depicting changes in bmp2, runx-2, alp and sparc mRNAs in PDPCs on PEGDA-AA/Arb AE Ann. cultured in complete (CM) or differentiating (DM) medium for 7 days. Data are expressed as fold change (2^−ΔΔCt) over PEGDA-AA Ann. The axes intersect at 1, which indicates the range of mRNA expression in cells cultured on PEGDA-AA Ann. (C) Histogram depicting changes in bmp2, runx-2, alp and sparc mRNAs in PDPCs on PEGDA-AA/Arb AE Ann. cultured in complete (CM) or differentiating (DM) medium both in the presence of H₂O₂ for 7 days. Data are expressed as fold change (2^−ΔΔCt) over PEGDA-AA Ann. in the presence of H₂O₂. The axes intersect at 1, which indicates the range of mRNA expression in untreated cells; # indicates significant differences (p < 0.05) in comparison to controls without arbutin and square brackets indicate significant differences between cells cultured in CM and DM. (D) Western blot analysis protein expression. (E) Histograms depicting the densitometric quantitation of RUNX-2 and ALP of the blots presented in D. Since BMP-2 and ON were not expressed, they were not considered for the histogram. (F) FoxO and β-catenin variations in PEGDA-AA Ann. and PEGDA-AA/Arb AE Ann. in cells undergoing stress induction. Data are expressed as fold change (2^−ΔΔCt) of H₂O₂ untreated cells; the axes intersect at 1, which indicates the range of mRNA expression in untreated cells.