Figure 3.

The up-regulation of GstO2 in motor neurons alleviated the motility deficits caused by hTDP-43. (a) Schematic illustration and quantification of larvae crawling traces from 60 s trials. The crawling distance of larvae overexpressing hTDP-43 decreased compared to the control larvae. Overexpression of GstO2 suppressed defective locomotive activity in hTDP-43-induced larvae. Overexpression of GstO2 alone had no effect on crawling activity. Statistical significance was determined using the unpaired Student t-test (* p < 0.05; *** p < 0.001, n > 10 for each genotypes). Error bars indicate SEM of three independent experiments. (b) The ratio of climbing activity in adult flies was grown at 29 °C. hTPD-43 expressed in motor neurons caused severe motility deficits in adult flies in an age-dependent manner. The overexpression of GstO2 in adult flies counteracted the reduction in climbing ability caused by hTDP-43 expression. The D42-Gal4 control flies and flies expressing GstO2 alone showed no defects in locomotion. Statistical significance was determined using the unpaired Student t-test (*** p < 0.001, n = 20 for each genotypes). Error bars indicate SEM of more than five independent experiments.