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. 2020 Jul 10;9(7):608. doi: 10.3390/antiox9070608

Figure 3.

Figure 3

Effects of GYY4137 on the rat DLNCs. The DLNCs were isolated from the rats immunized with MOG + CFA. The percentage of CD4+ CD25+ FoxP3+ cells (Treg), and the proportion of IL17+ among the CD4+ cells were determined by cytofluorimetry. The percentage of Treg was determined among the DLNCs (A) or among CD4+ T cells purified from the DLNCs (B), after 40 min of cultivation in the presence of 200 μM GYY4137 (GYY) or DMSO as the vehicle (DMSO). The percentage of Th17 cells was determined among the DLNCs, treated with GYY4137 (GYY) or DMSO as the vehicle (DMSO) for 40 min and subsequently stimulated for 4 h (C). In some experiments, the cultures without DMSO (Ctrl) were also performed (A,C). IL-17 and IFN-γ cytokine concentrations in the supernatants of the 24 h cell cultures of MOG re-stimulated DLNCs and treated with GYY4137, were determined by ELISA (D). Data are presented as the mean + standard deviation (SD) from three (C), four (B) or six samples (A,D). * p < 0.05 refers to DMSO.