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. 2020 Jul 10;9(7):608. doi: 10.3390/antiox9070608

Figure 4.

Figure 4

Effects of GYY4137 on rat spinal cord immune cells (SCICs). SCICs and DLNCs were isolated from rats immunized with rat spinal cord homogenate (SCH) + CFA at the peak of the disease or at day 7 post immunization, respectively. Clinical course of the EAE is presented (A). The percentage of the CD4+ CD25+ FoxP3+ cells (Treg), and the proportion of IL17+ among the CD4+ cells were determined by cytofluorimetry. The percentage of Treg was determined among the SCICs after 40 min cultivation in the presence of 200 μM GYY4137 (GYY) or DMSO as the vehicle (DMSO) (B) The percentage of IL-17+ cells among the CD4+ cells was determined among the SCICs (C) or among the CD4+ T cells purified from the SCICs (D), after 40 min cultivation in the presence of 200 μM GYY4137 (GYY) or DMSO as the vehicle (DMSO). The percentage of Treg was determined among the DLNCs after 40 min cultivation in the presence of 200 μM GYY4137 (GYY) or DMSO as the vehicle (DMSO) (E). The percentage of IL-17+ cells among the CD4+ cells was determined among the DLNCs (F) after 40 min cultivation in the presence of 200 μM GYY4137 (GYY) or DMSO as the vehicle (DMSO). In some experiments, the cultures without DMSO (Ctrl) were also performed (B,C). IL-17 and IFN-γ cytokine concentrations in the supernatants of 24 h cell cultures of SCICs treated with GYY4137 were determined by ELISA (G). Data are presented as the mean + standard deviation (SD) from three (C,D,F), four (B,E) or six (A,G) samples. * p < 0.05 refers to Ctrl.