Figure 3.

Rcor1 is dispensable for thrombopoiesis. (A): May-Grunwald Giemsa stained bone marrow (BM) touch preparations revealed normal megakaryocyte morphology in Rcor1^−/− mice. (B): vWF⁺ (red) megakaryocytes with a normal distribution in Rcor1^−/− BM. Tibia sections were counterstained with 4′,6-diamidino-2-phenylindole (blue). (C): Flow cytometry analysis of MkP. (D): MkP frequency was not significantly altered in Rcor1-deficient BM (n_mutant = 5, n_control = 3). (E): Typical acetylcholinesterase⁺ CFU-Mk derived from control and mutant BM cells. (F): The Rcor1^flox allele was not detectable in sorted MkPs after Cre induction. Scale bars = (A–B) 20 μm; (E) 100 μm. Abbreviation: MkP, megakaryocyte progenitors.