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. Author manuscript; available in PMC: 2021 May 22.
Published in final edited form as: J Nat Prod. 2020 Apr 29;83(5):1461–1472. doi: 10.1021/acs.jnatprod.9b01022

Figure 4.

Figure 4.

Immunoblot detection and quantification of ABCG2 protein in (A) ABCG2-overexpressing S1-M1–80 human colon cancer cells and (B) ABCG2-overexpressing H460-MX20 human lung cancer cells treated with DMSO (vehicle control) or increasing concentrations (0.5 – 3.0 μM) of licochalcone (LCA) for 72 h according to the method described previously.79 α-Tubulin was used as an internal loading control. Values are presented as mean ± SEM calculated from three independent experiments.